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Lei Lu
  • Faculty, School of Biological Sciences, Nanyang Technological University, Singapore
Research focus
  • Biochemistry, Cell Biology
Personal information

Education

PhD, National University of Singapore, 2004

Lab information

Lei Lu Lab
https://sites.google.com/site/leilulab/home-1

Research focus

Golgi, cilium, and imaging tool development.

Publications

https://sites.google.com/site/leilulab/publications-2
Shi M, Tie HC, Mahajan D, Sun X, Zhou Y, Boh BK, Vardy LA and Lu L. (2022) Arl15 upregulates the TGFβ family signaling by promoting the assembly of the Smad-complex. eLife 11:e76146.

Tie HC, Mahajan D, and Lu L. (2022) Visualizing intra-Golgi localization and transport by side-averaging Golgi ministacks. J. Cell Biol. 221(6):e202109114.

Sun X, Mahajan D, Chen B and Lu L. (2021) A quantitative study of the Golgi retention of glycosyltransferases. J. Cell Sci. 134(20).

JCS First Person - Xiuping Sun

Recommended in Faculty Opinions

Sun X, Tie HC, Chen B and Lu L. (2020) Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. J. Biol. Chem. 295(43).

Recommended in Faculty Opinions

Mahajan D, Tie HC, Chen B and Lu L (2019) Dopey1-Mon2 complex binds to dual-lipids and recruits kinesin-1 for membrane trafficking. Nature communications 10(3218).

Tie HC, Ludwig A, Sandin S and Lu L (2018) The spatial separation of processing and trafficking functions to the interior and periphery of the Golgi stack. eLife 7:e41301.

Recommended in Faculty Opinions.

Shi M , Chen B, Mahajan D, Boh BK, Zhou Y, Dutta B, Tie HC, Sze SK, Wu G and Lu L (2018) Amino acids stimulate theendosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nature Communications 9(4987).

Zhang H, Ba S, Mahajan D, Lee JY, Ye R, Shao F, Lu L# and Li T#. (2018) Versatile Types of DNA-Based Nanobiosensors for Specific Detection of Cancer Biomarker FEN1 in Living Cells and Cell-Free Systems. Nano Lett. doi: 10.1021/acs.nanolett.8b03724. (#Co-correspondence authors)

Lu L and Madugula V. (2017) Mechanisms ofciliary targeting — entering importins and Rabs. Cell Mol. Life Sci. https://doi.org/10.1007/s00018-017-2629-3.

Karthikeyan A, Gupta N, Tang C, Mallilankaraman K, Silambarasan M, Shi M, Lu L, Ang BT, Ling EA and Dheen ST. (2018) Microglial SMAD4 regulated by microRNA-146a promotes migration of microglia which supporttumor progression in a glioma environment. Oncotarget. 9(38): 24950-24969.

Tie HC, Chen B, Sun X, Cheng L and Lu L. (2017) Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass. J. Vis. Exp. (126), e55996.

Wang Y, Zhang H, Shi M, Liou YC, Lu L, Yu F. (2017) Sec71 functions as a GEF for the small GTPase Arf1 to govern dendrite pruning of Drosophila sensory neurons. Development. 15;144(10) 1851-1862.

Tie HC#, Madugula V# and Lu L (2016) The development of a single molecule fluorescence standard and its application in estimating the stoichiometry of the nuclear pore complex. Biochem. Biophys. Res. Commun. 478(4):1694-1699.

# Equal contribution.

Madugula V and Lu L. (2016) The ciliary membrane targeting by a ternary complex comprising transportin1, Rab8 and the ciliary targeting signal. J. Cell Sci. 129(20):3922-3934.

Winner of JCS prize 2016.

Tie HC, Mahajan D, Chen B, Cheng L, VanDongen AM, Lu L. (2016) A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargoes. Mol. Biol. Cell. 27:848-861. (cover image)

Mu, J., Liu, F., Rajab, M.S., Shi, M., Li, S., Goh, C., Lu, L., Xu, Q.H., Liu, B., Ng, L.G. and Xing, B. (2014) A small-molecule FRET reporter for the real-time visualization of cell-surface proteolytic enzyme functions. Angew Chem. Int. Ed. Engl. 53(52):14357-14362.

Lu, L. and Hong, W. (2014) (review) From endosomes to the trans-Golgi network. Semin. Cell Dev. Biol. 31:30-39.

Mahajan, D., Boh, B.K., Zhou, Y., Chen, L., Cornvik, T.C., Hong, W. and Lu, L (2013) Mammalian Mon2/Ysl2 regulates endosome-to-Golgi trafficking but possesses no guanine nucleotide exchange activity toward Arl1 GTPase Sci. Rep.3:3362.

Zhou, Y., Hong, W. and Lu, L. (2013) Imaging beads-retained prey assay for rapid and quantitative protein-protein interaction. Plos One 8: e59727

Lu, L., Hannoush, R., Goess, B., Varadarajan, S., Shair, M. and Kirchhausen, T. (2013) The small molecule dispergo tubulates the endoplasmic reticulum and inhibits export. Mol. Biol. Cell. 24:1020-1029.

Lu, L.# and Kirchhausen, T (2012) Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane. Commun. Integr. Biol. 5:1, 1-3.

(#correspondence author)

Lu, L.#, Ladinsky, M. S., and Kirchhausen, T#. (2011) Formation of the post-mitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly. J. Cell Biol. 194: 425-440.

(# Co-correspondence authors)

Cover image featured in JCB In Focus, Biosights and Journal Club.

Smits, P., Bolton, A.D., Funari, V., Hong, M., Boyden, E.D., Lu, L., Manning, D.K., Dwyer, N.D., Moran, J.L., Prysak, M., Merriman, B., Nelson, S,F., Bonafe, L., Superti-Furga, A., Ikegawa S., Krakow, D., Cohn, D.H., Kirchhausen, T., Warman, M.L. and Beier, D.R. (2010) Lethal Skeletal Dysplasia in Mice and Humans Lacking the Golgin GMAP-210. N. Engl. J. Med.

Lu, L., Ladinsky, M.S. and Kirchhausen, T. (2009) Cisternal organization of the endoplasmic reticulum during mitosis. Mol Biol Cell. 20:3471-3480.

Cover image and featured in Incyte from MBC.

Lu, L. and Hong, W. (2008) Retrograde endosome-to-TGN transport. The Golgi apparatus. State of art after 110 years of Camillio’s discovery. Mironov, A. and Pavelka, M. (eds). Chapter 3.8, pp 425-458.

Zahn, C., Jaschke, A., Weiske, J., Hommel, A., Hesse, D., Augustin, R., Lu, L., Hong, W., Florian, S., Scheepers, A., Joost, H.G., Huber, O. and Schürmann, A. (2008) ADP-ribosylation factor-like GTPase ARFRP1 is required for trans-Golgi to plasma membrane trafficking of E-cadherin. J. Biol. Chem. 283: 27179-27188.

Zahn, C., Hommel, A., Lu L., Hong, W., Walther, D.J., Florian, S., Joost, H. and Schurmann A. (2006) Knockout of Arfrp1 leads to disruption of ARF-like1 (ARL1) targeting to the trans-Golgi in mouse embryos and HeLa cells. Mol. Mem. Bio., 23: 475-485.

Lu, L., Tai, G., Wu, M., Song, H., and Hong, W. (2006) Multilayer interactions determine the Golgi localization of GRIP golgins. Traffic. 7:1399-13407.

Wang, Y., Tai, G., Lu, L., Johannes, L., Hong, W., and Tang, B. (2005) Trans-Golgi network syntaxin 10 functions distinctly from syntaxins 6 and 16. Mol. Membr. Biol. 22:313-325.

Tai, G., Lu, L., Johannes, L., and Hong, W. (2005) Functional analysis of Arl1 and Golgin-97 in endosome-to-TGN transport using recombinant Shiga toxin B fragment. Method. Enzymol. 404:442-453.

Lu, L., Tai, G., and Hong, W. (2005) Interaction of Arl1 GTPase with the GRIP domain of Golgin-245 as assessed by GST (glutathione-S-transferase) pull-down experiments. Method. Enzymol. 404:432-441.

Derby, M.C., van Vliet, C., Brown, D., Luke, M.R., Lu, L., Hong, W., Stow, J.L., and Gleeson, P.A. (2004) Mammalian GRIP domain proteins differ in their membrane binding properties and are recruited to distinct domains of the TGN. J. Cell Sci. 117:5865-5874.

Tai, G., Lu, L., Wang, T.L., Tang, B.L., Goud, B., Johannes, L. and Hong, W. (2004) Participation of the syntaxin 5/Ykt6/GS28/GS15 SNARE complex in transport from the early/recycling endosome to the trans-Golgi network. Mol. Biol. Cell, 15: 4011-4022.

Wang, C., Ng, C., Lu, L., Atlashkin, V., Zhang, W., Seet, L., and Hong, W. (2004) A role of VAMP8/Endobrevin in regulated exocytosis of pancreatic acinar cells. Dev. Cell, 7: 359–371.

Lu, L.#, Tai, G.#, Hong, W.J. (2004) Autoantigen Golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the TGN. Mol. Biol. Cell, 15, 4426-4443.

(#: these two authors contributed equally to this work)

Wu, M.#, Lu, L.#, Hong, W.J., and Song, H. (2004). Structural basis for recruitment of GRIP domain golgin-245 by small GTPase Arl1. Nat. Struct. Mol. Biol. 11, 86-94.

(#: these two authors contributed equally to this work)

Lu, L., and Hong,W.J. (2003). Interaction of Arl1-GTP with GRIP domains recruits autoantigens Golgin-97 and Golgin-245/p230 onto the Golgi. Mol. Biol. Cell 14, 3767-3781.

Lu, L., Horstmann,H., Ng,C., and Hong,W.J. (2001). Regulation of Golgi structure and function by ARF-like protein 1 (Arl1). J. Cell Sci. 114, 4543-4555.

Bui, T.D., Wong, S.H., Lu, L. and Hong, W.J. (1998) Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse, Genomics, 54, 579-580.
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