What is the difference with single cell imaging?

thats an interesting question. Single-molecule localization microscopy (SMLM) is based on the separation of the point spread function (PSF) of multiple individual emitters. This can be achieved by several mechanisms, e.g temporal separation (photo-conversion/switching/activation) of neighboring emitters or intermittent binding of the emitter to the molecule of interest), leading to a spatial resolution of ~20-50nm, far better than the ~200nm of conventional confocal microscopy. A variety of microscopes can be used with this approach, including confocal microscopes, TIRF microscopy and light-sheet microscopy, as long as the signal to noise ratio is permissible.