What is single-molecule microscopy?

Single-molecule localization microscopy (SMLM) requires a separation of the point spread function (PSF) of multiple individual emitters, which can be achieved by e.g. temporal separation (photo-conversion/switching/activation of neighboring emitters or intermittent binding of the emitter to the molecule of interest) and can enable spatial resolution of ~20-50nm. A variety of microscopes can be employed, including confocal microscopes, TIRF microscopy and light-sheet microscopy, as long as the signal to noise ratio is permissible. (e.g.https://bio-protocol.org/e4390).