What are commonly used fluorophores/dyes in super-resolution microscopy?

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I want to use super-resolution microscopy for my project, and am looking at different options to visualize my protein. can you help me with that?

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Wendy Stevens Answered Aug 6, 2022

Pennsylvania State University College of Medicine

The choice of fluorophores/dyes used for super-resolution microscopy depends on the technique utilized. For example, STORM and PALM utilize sequential activation of photoswitchable fluorophores to build a time-resolved, high-resolution image and thus requires the use of photoswitchable fluorophores or dyes, such as Alexa-Fluor dyes with different wavelength specs which can be conjugated to an antibody or other protein for imaging. STED, which selectively deactivates fluorophores, can and commonly use Alexa-Fluors, but can also utilize fluorescent proteins such as GFP, or CLIP-/SNAP-tags for in vivo imaging. For SIM, which combines use of a projected periodic grid to create interference patterns with deconvolution, both synthetic dyes such as Alexa-Fluor and genetically encoded fluorophores such as GFP can be used. 

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