What is the basic principle of the microscopy?

HI Chenchen Liu,

this webinar focusses on single-molecule localization microscopy (SMLM), which is one of several super resolution microscopy techniques. It can enable spatial resolution of up to ~20-50nm, which is better than the ~200nm resolution of conventional confocal microscopy. Single-molecule microscopy is based on the ability to separate multiple individual emitters in close proximity. This separation can be accomplished by several mechanisms, including temporal separation (photo-conversion/switching/activation) of neighboring emitters or intermittent binding of the emitter to the molecule of interest. You can use different microscopes with this approach, including confocal microscopes, TIRF microscopy and light-sheet microscopy, as long as the signal to noise ratio is permissible.