Preparation of Taq DNA Polymerase

Materials and Reagents

1. Dialysis tubing cellulose membrane avg. flat width 33 mm (Sigma-Aldrich, catalog number: D9652 )
2. E.coli BL21 strain 
3. Ammonium sulfate (Sigma-Aldrich, catalog number: A4418 )
4. IPTG
5. Ampicillin
6. (NH₄)₂SO₄
7. 1 M dextrose
8. 1 M Tris - HCl (pH 7.9)
9. 0.5 M EDTA (pH 8.0)
10. KCl
11. PMSF
12. Tween 20
13. NP40
14. Lysozyme
15. Glycerol
16. Triton-X 100
17. Lysis buffer (see Recipes)
18. Buffer A (see Recipes)
19. Pre - lysis buffer (see Recipes)
20. Storage buffer (see Recipes)
21. TAQ buffer (see Recipes)
22. MgCl₂ solution (see Recipes)
    

Equipment

1. 2 L flask
2. Centrifuges
3. Oakridge tubes
   

Procedure