What is the length of RNA that can be sequenced? Can one determine the transcriptome of single cells in a culture having mutants & revertants?

I have a mutant that reverts very fast or frequently accumulates suppressor mutations thereby making it very difficult to obtain a stable homogenous mutant culture. This mutant inhibits the expression of sugar operons and the cyaA gene in E. coli. I want to determine the transcriptomics Read more Read less