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I want to use this nano-body mediated E3 ligase technique to edit in plant (knockout gene) will you please let me know in brief how to do it?

MM
MUHAMMAD ZEESHAN MOLA BAKHSH Jun 7, 2024
webinar Webinar: From Llama to Nanobody: A Streamlined Workflow for the Generation of Functionalised VHHs

i want to mutate a gene through this nano-body E3 ligase by using some GFP. i have no idea how to use it and don't find any suitable paper for this. please help me out thank you.
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Lauren Eyssen Answered Jun 13, 2024

Rosalind Franklin Institute

Hi Muhammad

Pleas have a look at this paper to start your search into this method.

Antibody RING-Mediated Destruction of Endogenous Proteins: Molecular Cell


References

1.
10.1016/j.molcel.2020.04.032
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