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Is it ok to use pAPIII6 vector for nanobody biopanning and expression,and what is the suitable medium for expression?

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Roaa Khaled El-Sayed May 30, 2024
webinar Webinar: From Llama to Nanobody: A Streamlined Workflow for the Generation of Functionalised VHHs
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Lauren Eyssen Answered Jun 5, 2024

Rosalind Franklin Institute

The pAPIII6 vector is a phagemid vector (it has the bacteriophage minor capsid protein) and can be used. It has been used in literature for scFvs but can be used for VHHs


References

1.
https://doi.org/10.1016/S0022-1759(01)00463-X
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