Whatkind of samples can be used in this methodology? Any precautions we might have before including this methodology in our projects?

webinar Webinar: Developing glycoproteomics and glycoengineering approaches to investigate protein glycosylation

What are the necessary equipments and supplies for a lab to implement this methodology? And for the sample? Is it possible to perform in frozen plant extracts samples?

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Lorenzo Povolo Answered Apr 8, 2024

University of Copenhagen

Thank you for your question! In theory, any sample you can extract proteins from can be used as an input for glycoproteomics workflows. Typically these could be cell pellets, tissues, organs or even entire organisms (flies, zebrafish, etc.). For more complex samples you might need to homogenize the input sample for a optimal solubilization with detergents. I sadly do not have experience with plant cells, but my guess is that it is possible to adapt published protocols (mainly developed for eukaryotic cells) to plant cells. Regarding the equipment/supplies, we will go through this part during the webinar, but please feel free to reach out after the presentation should anything remain unclear.

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