Lorenzo Povolo Answered Apr 8, 2024
University of Copenhagen
Thank you for the question! Main workflows for proteome-wide glycoproteomics are rather versatile and could indeed be used to elucidate if glycosylation changes are associated with cell dynamics. For this aim, you could consider using quantitative MS/MS approaches based on protein- (eg. SILAC) or peptide-level labelling (eg. dimethyl, diethyl, TMT,...) to compare the glycosylation status between two (or more) conditions, treatments and so on. The attached reference could give you an example of how glycoproteomics (combined with glycomics in this case) can be applied in a similar setup. I hope this can be of help, please do not hesitate to raise the point during the Q&A session!
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