Contig interaction frequency and local assembly

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Rui Cao
May 11, 2023
protocol Protocol: An optimized four-step genome assembly strategy

Dear Dr Tian,


Thanks for your sharing. I still have two question for this pipeline:

(1) how to caculated the interaction frequency: I see in your method, you use bowtie2 and HiCUP pipeline in step 2, I wonder which output files can generate linkage frequency? Once Read more Read less more less

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Shilin Tian Author Answered May 14, 2023

Wuhan University

(1) You can use the "extract" command in the software AllHiC to perform the agglomerative hierarchical clustering algorithm.

(2) In Step 3, the optimal alignment reads for each linkage group were obtained, and you can perform local assembly of the ONT reads for each linkage group.

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