The Nature Protocol UMI labelling appears to be pseudo-barcoding

Graham Johnson
Graham Johnson
Apr 22, 2023
protocol Protocol: Cell line infection with lentiMPRA library, RNA- and DNA-seq, and read processing

As described the UMI labelling step appears to be pseudo molecular barcoding. In each of the three PCR cycles the template DNAs will anneal a different UMI primer. The progeny molecules from the same template will be labelled by a different UMI inflating estimates of the original abunda Read more Read less more less

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