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Genetic labeling and pre- and post-sequencing quality checks of SGNs, HCs, and glial cells, and library construction

ZL Zhiyong Liu

Last updated date: Mar 31, 2020 Views: 1264 Forks: 0

original An abbreviated version of this protocol was published in eLife in Jan, 2020
Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages
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All the raw data of our RNA-seq have been deposited in GEO (Gene Expression Omnibus) under the accession: GSE132925. The read counts of all the genes are available in the file: GSE132925-cochlear-SGN-cnts.txt.gz attached in GEO. 


here is the link: https://www.ncbi.nlm.nih.gov/gds/?term=GSE132925 


Any more questions, please email me at Zhiyongliu@ion.ac.cn 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Liu, Z(2020). Genetic labeling and pre- and post-sequencing quality checks of SGNs, HCs, and glial cells, and library construction. Bio-protocol Preprint. bio-protocol.org/prep257.
  2. Li, C., Li, X., Bi, Z., Sugino, K., Wang, G., Zhu, T. and Liu, Z.(2020). Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages. eLife. DOI: 10.7554/eLife.50491

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