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Preprint

Slingshot

BM Bryan Marsh
RB Robert Blelloch

Last updated date: Mar 24, 2022 Views: 634 Forks: 0

original An abbreviated version of this protocol was published in eLife in Nov, 2020
Single nuclei RNA-seq of mouse placental labyrinth development
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Slingshot was used according to instructions from the developers found here: https://bioconductor.org/packages/devel/bioc/vignettes/slingshot/inst/doc/vignette.html 

https://github.com/kstreet13/slingshot

 

Attached is the R code used perform slingshot on the integrated trophoblast dataset to perform trajectory inference from LaTP to the SynTI, SynTII, and S-TGC. The input files as R objects can be downloaded here: https://figshare.com/projects/Single_nuclei_RNA-seq_of_mouse_placental_labyrinth_development/92354

Related files

txt mouse.troph.combined.slingshot.txt download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Marsh, B and Blelloch, R(2022). Slingshot. Bio-protocol Preprint. bio-protocol.org/prep1598.
  2. Marsh, B. and Blelloch, R.(2020). Single nuclei RNA-seq of mouse placental labyrinth development. eLife. DOI: 10.7554/eLife.60266

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