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Immunofluorescence staining and fluorescence in situ hybridization (FISH)

HX Hong Xu
ZW Zong-Heng Wang

Last updated date: Sep 20, 2021 Views: 858 Forks: 0

original An abbreviated version of this protocol was published in eLife in Oct, 2019
Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila
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Hello,

 

I am the first author of this paper. We used a generic protocol for immunofluorescence staining. Detailed procedure for FISH is conducted according to Nature Protocols 12:1326–1348. Please find this paper in the attachment of this email.

 

Thanks,

 

Zong-Heng

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pdf nprot.2017.030.pdf download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Xu, H and Wang, Z(2021). Immunofluorescence staining and fluorescence in situ hybridization (FISH). Bio-protocol Preprint. bio-protocol.org/prep1374.
  2. Wang, Z., Liu, Y., Chaitankar, V., Pirooznia, M. and Xu, H.(2019). Electron transport chain biogenesis activated by a JNK-insulin-Myc relay primes mitochondrial inheritance in Drosophila. eLife. DOI: 10.7554/eLife.49309

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