Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Generation of transgenic lines

SC Stephanie J Conway
JL Jane A Langdale
VD Verónica S Di Stilio

Last updated date: Aug 31, 2021 Views: 705 Forks: 0

original An abbreviated version of this protocol was published in eLife in Oct, 2018
LEAFY maintains apical stem cell activity during shoot development in the fern Ceratopteris richardii
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite

The protocols for transgenic fern were published in 2014, 2015, first as an article, then as a protocol. Both are Open access

 

Plackett AR, Huang L, Sanders HL, Langdale JA. High-efficiency stable transformation of the model fern species Ceratopteris richardii via microparticle bombardment. Plant Physiol. 2014 May;165(1):3-14. doi: 10.1104/pp.113.231357. Epub 2014 Mar 12. PMID: 24623851; PMCID: PMC4012588.

 

Plackett, A.R.G., Rabbinowitsch, E.H. & Langdale, J.A. Protocol: genetic transformation of the fern Ceratopteris richardii through microparticle bombardment. Plant Methods 11, 37 (2015). https://doi.org/10.1186/s13007-015-0080-8

Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Conway, S, Langdale, J and Di Stilio, V(2021). Generation of transgenic lines. Bio-protocol Preprint. bio-protocol.org/prep1355.
  2. Plackett, A. R., Conway, S. J., Hewett Hazelton, K. D., Rabbinowitsch, E. H., Langdale, J. A. and Di Stilio, V. S.(2018). LEAFY maintains apical stem cell activity during shoot development in the fern Ceratopteris richardii . eLife. DOI: 10.7554/eLife.39625

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A
This protocol preprint was submitted via the "Request a Protocol" track.