Materials and Reagents

1. Fluorescent micro beads (e.g. FluoSpheres 0.5 μm) (Life Technologies, catalog number: F-8813 )
   
2. Agarose
   
3. Penicillin/Streptomycin (10.000 Units/ml; Gibco^®, catalog number: 15140 )
   
4. HEPES Pufferan (Carl Roth, catalog number: HN78.3 )
   
5. Bead solution (see Recipes)
   
6. 5x MBSH (Modified Barth‘s Saline) (see Recipes)
   

Equipment

1. Sharp forceps (Fine Science Tools, catalog number: Dumont #5 )
   
2. Micro blade (Fine Science Tools, Dissecting Knife - Fine Tip)
   
3. Sectioning dish (Petri Dish with bottom covered in 1% Agarose in 1x MBSH)
   
4. Transfer pipet (Carl Roth, model: EA65.1 )
   
5. Glass staining block (Karl-Hecht Assistent (Lymphbecken) 2020)
   
6. Microscope slides
   
7. Coverslips
   
8. 5 ml syringe
   
9. Vaseline
   
10. Fluorescence microscope equipped with a digital camera (wide field)
    
11. Large Petri dish filled with PBS or MBSH for explant retrieval
    

Procedure