Materials and Reagents

1. Arabidopsis seedlings
   
2. Milli-Q water
   
3. Nitric acid (analytical grade for heavy metals) (Wako Pure Chemical Industries, catalog number: 140-04016 )
   
4. Potassium standard solution (1,000 ppm) (Wako Pure Chemical Industries, catalog number: 165-17471 )
   
5. Contaminon L (Wako Pure Chemical Industries, catalog number: 035-09311 )
   
6. Murashige and Skoog salt (Wako Pure Chemical Industries, catalog number: 392-00591 )
   
7. 2-(N-morpholino)ethanesulfonic acid (MES, Wako Pure Chemical Industries, catalog number: 345-01625 )
   
8. Gamborg’s Vitamin Solution (Sigma-aldrich, catalog number: G1019 )
   
9. Difco Bacto agar (Becton, Dickinson and Company, catalog number: 214010 )
   
10. Germination media (GM)-agar (see Recipes)
    

Equipment

1. Plates
   
2. Centrifugation tubes
   
3. A paper or aluminum foil
   
4. Atomic absorption spectrometer (AAS) or Inductively-coupled plasma optical emission spectrometer (ICP-OES)
   
5. Stainless-steel vessel (HU-25, o.d. = 55 mm x 104 mm) (SAN-AI science)
   
6. Polytetrafluoroethylene (PTFE) vessel (o.d. = 28 mm x 40 mm) (SAN-AI science)
   
7. Tetrafluoroethylene-perfluoroalkyl vinyl ether copolymer (PFA) cup (o.d. = 22 mm x 32 mm, 7 ml) (Sanplatec, catalog number: 0225R )
   
8. Volumetric flask (100 ml)
9. Ceramic scissors and tweezers (AS ONE Corporation, catalog number: 8-203-23 , 7-166-11 )
   

Procedure