Materials and Reagents

1. EDTA blood (extracted blood kept in tubes with EDTA for anticoagulant purposes)
   
2. PBS
   
3. Ficoll-Hypaque solution (density 1.077 g/L, Ficoll-Paque PLUS) (General Electric Company, catalog number: 17-1440-03 )
   
4. FBS
   
5. Complete RPMI-10 (RPMI, 10% FBS)
   

Equipment

1. 15- or 50 ml conical centrifuge tube
   
2. Beckman GPR centrifuge with GH-3.7 horizontal rotor (or equivalent temperature controlled centrifuge)
   

Procedure