内生真菌提取物的生物检测

Susheel  Kumar; Nutan  Kaushik

doi:10.21769/BioProtoc.927

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Peer-reviewed

Bioassay of Extracts of the Endophytic Fungi

内生真菌提取物的生物检测

Susheel Kumar

Nutan Kaushik email

发布: 2013年10月05日第3卷第19期 DOI: 10.21769/BioProtoc.927 浏览次数: 9907

评审: Ru Zhang

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The authors used this protocol in:

Cover of PLOS ONE, featuring study using the protocol.

Feb 2013

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Abstract

Many of the microbes including fungi produce metabolites possessing antifungal activity and in some cases fungal metabolites are main cause of antifungal activity resulted by fungus. To infer that antifungal activity is due to fungal metabolites, there is a need to develop a repeatable procedure to assay these metabolites. Here we are presenting the poisoned food technique for bioassay of extract from endophytic fungi, where culture media is supplemented with extract to testpathogen viability as proxy for antifungal activity.

Keywords: Bioassay (生物测定)