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Batch Culture Fermentation of Endophytic Fungi and Extraction of Their Metabolites

内生真菌的分批培养发酵及其代谢物的提取

Susheel Kumar Susheel Kumar
Nutan Kaushik Nutan Kaushik

发布: 2013年10月05日第3卷第19期 DOI: 10.21769/BioProtoc.926 浏览次数: 15868

评审: Ru Zhang

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Abstract

Antibiosis is one of the possible modes of action shown by endophytic fungi having antifungal activity. To test if antifungal activity in endophytic fungi is due to antibiosis, assay of the metabolites of endophytic fungi was needed. To obtain metabolites for bioassay batch culture fermentation and extraction of metabolites was done. Fungus was multiplied on wickerham media at incubation temperature of 25 ± 2 °C for 4 weeks and then extracted with solvents of different polarity. All the solvent extracts were dried under vacuum rotary evaporator to get dried crude fungal extract, which was subjected to further fractionation and bioassay.

Keywords: Fermentation (发酵) search Endophytic fungi (内生真菌) search Batch culture (批文化) search Metabolites of endophytic fungi (内生真菌metabolites of) search Solvent extraction (溶剂萃取) search

Materials and Reagents

  1. Ethyl acetate (Rankem)
  2. Butanol (Qualigens)
  3. Methanol (Qualigens)
  4. Hexane (Qualigens)
  5. Fungus culture
  6. Measuring cylinder
  7. Whatman filter paper
  8. Detergent
  9. Glass jars of 5 L capacity
  10. Malt extract (HiMedia Laboratories)
  11. Yeast extract (HiMedia Laboratories)
  12. Peptone (HiMedia Laboratories)
  13. Glucose (Qualigens)
  14. Needle
  15. Spirit lamp
  16. Commercial disinfectant (70% ethanol)
  17. Vacuum pump
  18. Malt extract
  19. Yeast extract
  20. Wickerham medium (see Recipes)

Equipment

  1. Inoculation flasks
  2. Conical flasks of 1 L capacity
  3. Vacuum rotary evaporator (Heidolph Instruments GmbH)
  4. pH meter (Eutech Instruments pH tutor)
  5. Autoclave (Nat steel)
  6. BOD incubator (Toshiba)
  7. Laminar air flow hood (Toshiba)
  8. Filteration assembly 
  9. Hand blender (inalsaappliances.com)
  10. Fume hood
  11. Microbalances (Sartorious, model: RC210P )

Procedure

English
中文翻译

文章信息

版权信息

© 2013 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Kumar, S. and Kaushik, N. (2013). Batch Culture Fermentation of Endophytic Fungi and Extraction of Their Metabolites. Bio-protocol 3(19): e926. DOI: 10.21769/BioProtoc.926.

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分类

微生物学 > 微生物细胞生物学 > 细胞分离和培养

生物化学 > 其它化合物 > 抗菌剂

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