ROR1 Flow Cytometry
ROR1 流式细胞检测
发布: 2013年09月20日第3卷第18期 DOI: 10.21769/BioProtoc.905 浏览次数: 8537
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参见作者原研究论文
The authors used this protocol in:
Nov 2012
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Abstract
ROR1 is a receptor tyrosine kinase family member studied for its roles in development and cancer. Here we describe a protocol for analysis of ROR1 surface expression in acute lymphoblastic leukemia immortalized cell lines by flow cytometry.
Materials and Reagents
- Cells (e.g. RCH-ACV cells, Kasumi-2 cells, REH cells, MHH-CALL-2 cells)
- FBS
- Antibody specific for ROR1 (R&D Systems, catalog number: AF2000 )
- Goat IgG (R&D Systems, catalog number: AB-108-C )
- Donkey Anti-goat IgG-Phycoerythrin (R&D Systems, catalog number: F0107 )
Equipment
- Centrifuge
- FACSAria (BD Biosciences)
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Bicocca, V. T. and Tyner, J. W. (2013). ROR1 Flow Cytometry. Bio-protocol 3(18): e905. DOI: 10.21769/BioProtoc.905.
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分类
细胞生物学 > 基于细胞的分析方法 > 流式细胞术
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