Materials and Reagents

1. C57BL/6 Mouse, 8 weeks old
   
2. Baytril (Enrofloxacin) Antibacterial injectable solution 2.27% (Bayer HealthCare)
   
3. Buprenex (Buprenorphine hydrochloride) stock (0.3 mg/ml) (Reckitt Benckiser Healthcare)
   
4. Isothesia (Isoflurare, USP) (Butler Schein)
   
5. 0.9% sodium chloride (NaCl) solution
   
6. 100% oxygen (O₂)
   
7. Surgical iodine
   
8. 70% Ethanol
   
9. 0.015 mg/ml Baytril in 0.9% NaCl (see Recipes)
   
10. 0.01 mg/ml Buprenex in 0.9% NaCl (see Recipes)
    

Equipment

1. IH-0400 Impactor (Precision Systems and Instrumentation, LLC)
   
2. 1.25 mm Impactor Tip
   Note: This is the standard-size mouse tip. A 2.5 mm tip size is available for rat procedures. The company can also make custom sized tips.
   
3. PC running Windows 95+ with 9-pin serial port
   
4. Sterile cotton tip applicators
   
5. Razor blades
   
6. Graefe micro dissecting tissue forceps (Roboz, catalog number: RS-5150 )
   
7. Noyes micro dissecting spring scissors (Roboz, catalog number: RS-5676 )
   
8. Operating scissors (Pakistan Scissors Industries, catalog number: PS-13-120 )
   
9. AUTOCLIP 9 mm wound clips (BD Biosciences) and applier
   
10. VetEquip rodent anesthesia machine
    
11. Electrical heat pad
    
12. Hamilton SafeAire laminar flood hood (Fisher Hamilton)
    

Procedure