水稻NO3-吸收量的测定-SITE法（scanning ion-electrode technique)

Zhong  Tang; Guohua  Xu

doi:10.21769/BioProtoc.876

Improve Research Reproducibility A Bio-protocol resource

提交稿件
订阅
CN
- EN - English
- CN - 中文

Peer-reviewed

Measurement of Net NO₃^- Flux in Rice Plants with the SIET System

水稻NO3-吸收量的测定-SITE法（scanning ion-electrode technique)

Zhong Tang

Guohua Xu email

发布: 2013年08月20日第3卷第16期 DOI: 10.21769/BioProtoc.876 浏览次数: 7979

评审: Tie Liu

下载 PDF

Q&A

引用

Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Plant Physiology, featuring study using the protocol.

Dec 2012

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

相关实验方案

拟南芥铵含量的高通量测定

Izargi Vega-Mas [...] Daniel Marino

2015年08月20日 10859 阅读

通过DFMA 和 DFMO 抑制番茄中腐胺的生物合成

Emma Fernández-Crespo [...] Gemma Camañes

2016年11月05日 8575 阅读

离子渗漏实验定量分析湿度对超敏反应的影响

Musoki Mwimba and Xinnian Dong

2019年04月05日 5365 阅读

Abstract

SIET (scanning ion-electrode technique) is a new technique to study the flow rate of the ions and molecules in real time in living biomaterials by using microelectrodes and microsensor. This technique allows non-invasive, simultaneous measurement of fluxes of specific ions at the surface of an intact plant. It has high temporal and spatial resolutions. This protocol uses the SIET system for the measurement of ions flux rate in rice plants.

Keywords: Nitrate transporter (硝酸盐转运蛋白)

Materials and Reagents

Rice seedlings: Three weeks old seedlings
Plastic supporting netting
Tributylchlorosilane (Fluka, catalog number: 90796 )
NH₄NO₃
KH₂PO₄
K₂SO₄
CaCl₂^.2H₂O
MgSO₄^.7H₂O
Na₂SiO₃
NaFeEDTA
H₃BO₃
MnCl₂^.4H₂O
CuSO₄^.5H₂O
ZnSO₄^.7H₂O
Na₂MoO_4^.2H₂O
International Rice Research Institute (IRRI) nutrient solution (see Recipes)
Calibrate solution (see Recipes)
Measuring solution (see Recipes)

Equipment

Small plastic dish (6 cm diameter)
Measuring chamber
Ion-selective electrodes (Clark Electromedical, model: GC150-10 )
BIO-IM (NMT-YG-100, Younger USA LLC, Amherst, model: MA01002 ) with ASET 2.0

Software

ASET 2.0 (Sciencewares, Falmouth, catalog number: MA 02540)
iFluxes 1.0 (YoungerUSA, LLC, Amherst, catalog number: MA 01002) software

Procedure

English

中文翻译

文章信息

版权信息

如何引用

Readers should cite both the Bio-protocol article and the original research article where this protocol was used:

Tang, Z. and Xu, G. (2013). Measurement of Net NO₃^- Flux in Rice Plants with the SIET System. Bio-protocol 3(16): e876. DOI: 10.21769/BioProtoc.876.
Tang, Z., Fan, X., Li, Q., Feng, H., Miller, A. J., Shen, Q. and Xu, G. (2012). Knockdown of a rice stelar nitrate transporter alters long-distance translocation but not root influx. Plant Physiol 160(4): 2052-2063.