采用REMA孔板法进行柑桔溃疡病亚型的药敏试验

Isabel C. Silva; Henrique Ferreira

doi:10.21769/BioProtoc.861

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Peer-reviewed

Drug Sensitivity Assay of Xanthomonas citri subsp. citri Using REMA Plate Method

采用REMA孔板法进行柑桔溃疡病亚型的药敏试验

Isabel C. Silva email

HF Henrique Ferreira

发布: 2013年08月20日第3卷第16期 DOI: 10.21769/BioProtoc.861 浏览次数: 10336

评审: Fanglian He

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Cover of Journal of Bacteriology, featuring study using the protocol.

Jan 2013

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Abstract

Resazurin Microtiter Assay (REMA) is a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. Resazurin detects cell viability by converting from a nonfluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth (Palomino et al., 2002). The REMA assay can be used as a fluorogenic oxidation-reduction indicator in a variety of cells, including bacteria, yeast and eukaryotes (Silva et al., 2013).

Keywords: Citrus canker (柑橘溃疡病)

Plant pathogen (植物病原菌)

Antibacterial (抗菌)

Materials and Reagents

Chemicals: Synthetic esters of gallic acids (Ximenes et al., 2010)
Bacterial strain: Wild type Xanthomonas citri subsp citri strain 306 (Schaad et al., 2005)
Dimethyl sulfoxide (DMSO) (Sigma-Aldrich, catalog number: D8418 )
Kanamycin (Sigma-Aldrich, catalog number: K4000 )
Luria-Bertani broth (LB) culture medium
Resazurin sodium salt (Sigma-Aldrich, catalog number: R7017 )

Equipment

96-well plate, polystyrene, with clear flat bottom wells (Greiner Bio-one, catalog number: 655101 )
SPECTRAfluor Plus (Tecan) microfluorimeter
Multichannel pipetman (Eppendorf)

Procedure

English

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如何引用

Silva, I. C. and Ferreira, H. (2013). Drug Sensitivity Assay of Xanthomonas citri subsp. citri Using REMA Plate Method. Bio-protocol 3(16): e861. DOI: 10.21769/BioProtoc.861.