Improve Research Reproducibility A Bio-protocol resource
Peer-reviewed

Preparation of Candida albicans Biofilms for Transmission Electron Microscopy

制备白色念珠菌生物膜用于透射电镜分析

HT Heather T. Taff
David R. Andes David R. Andes

发布: 2013年07月20日第3卷第14期 DOI: 10.21769/BioProtoc.822 浏览次数: 10799

评审: Fanglian He

download pdf PDF PDF
ask Q&A
引用
收藏
Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of PLOS Pathogens, featuring study using the protocol.

Aug 2012

Presubmission Inquiry

实验方案合集

专注于特定技术或应用的、详细的、经过同行评审的实验方案合集

Cancer Research
Immunology
Developmental Biology
Microbiology
Neuroscience
Cell Imaging - A Special Collection for Cell Bio 2023
查看更多 more

相关实验方案

利用 Percoll<sup>®</sup>在微分干涉显微镜下对隐球菌荚膜成像

利用 Percoll®在微分干涉显微镜下对隐球菌荚膜成像

Hugo Costa Paes [...] André Moraes Nicola

2019年11月20日 3646 阅读

白念珠菌生物膜细胞内cAMP的测定

白念珠菌生物膜细胞内cAMP的测定

Liuliu Jiang [...] Xin Wei

2019年12月05日 3982 阅读

真菌生物膜研究的新型稳健方法

真菌生物膜研究的新型稳健方法

Biswambhar Biswas [...] Anil Thakur

2025年01月05日 1235 阅读

Abstract

Transmission Electron Microscopy is a form of microscopy that allows for imaging of distinct portions of an individual cell. For Candida albicans biofilms, it is often used to visualize the cell walls of fixed samples of yeast and hyphae. This protocol describes how to grow, harvest, and fix Candida albicans biofilms in preparation for Transmission Electron Microscopy.

Materials and Reagents

  1. Plate containing grown Candida albicans colonies
  2. 500 ml 0.22 μm filter (Corning, catalog number: 431118 )
  3. Aluminum foil
  4. Bacto-peptone (BD Biosciences, catalog number: 211677 )
  5. Bacto-yeast extract (BD Biosciences, catalog number: 212750 )
  6. Cell scraper (BD Biosciences, Falcon®, catalog number: 353085 )
  7. Difco Dextrose (BD Biosciences, catalog number: 215530 )
  8. Glutaraldehyde (Sigma-Aldrich, catalog number: G5882 )
  9. Lead nitrate (PbNO3) (Electron Microscopy Sciences, catalog number: 17900 )
  10. MOPS (Thermo Fisher Scientific, catalog number: BP308 )
  11. 4% Osmium tetroxide (Electron Microscopy Sciences, catalog number: 19140 )
  12. Parafilm
  13. Paraformaldehyde (Electron Microscopy Sciences, catalog number: 15710 )
  14. Propylene oxide (Electron Microscopy Sciences, catalog number: 20410 )
  15. RPMI 1640 (Sigma-Aldrich, catalog number: R6504 )
  16. Sodium cacodylate trihydrate (Sigma-Aldrich, catalog number: C0250 )
  17. Sodium chloride (Thermo Fisher Scientific, catalog number: S671-3 )
  18. Sodium citrate (Electron Microscopy Sciences, catalog number: 21140 , 500 gm)
  19. Sodium hydroxide (Sigma-Aldrich, catalog number: 59223C )
  20. Sodium phosphate dibasic anhydrous (Thermo Fisher Scientific, catalog number: BP332-500 )
  21. Sodium phosphate monobasic monohydrate (Thermo Fisher Scientific, catalog number: S369-500 )
  22. Spurr’s Resin Kit (Polyscience, catalog number: 01916-1 )
  23. Uridine (Sigma-Aldrich, catalog number: U3750 )
  24. Uranyl acetate (Electron Microscopy Sciences, catalog number: 22400 )
  25. YPD + uridine (see Recipes)
  26. RPMI + MOPS (see Recipes)
  27. 1x PBS (see Recipes)
  28. Fixative (see Recipes)
  29. Reynold’s Lead Citrate Strain (see Recipes)

Equipment

  1. 15 ml conical tube (Corning, catalog number: CLS430055 )
  2. 6 well polystyrene plate (Grenier Bio-one, catalog number: 657160 )
  3. Hemocytometer
  4. Shaking incubator with adjustable temperatures and speeds
  5. Transmission Electron Microscope facility with trained microscopist

Procedure

English
中文翻译

文章信息

版权信息

© 2013 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Taff, H. T. and Andes, D. R. (2013). Preparation of Candida albicans Biofilms for Transmission Electron Microscopy. Bio-protocol 3(14): e822. DOI: 10.21769/BioProtoc.822.

ris ris Download Citation in RIS Format

分类

微生物学 > 微生物生物膜 > 生物膜培养

微生物学 > 微生物细胞生物学 > 细胞成像

细胞生物学 > 细胞成像 > 电子显微镜

您对这篇实验方法有问题吗？

在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。

0/150

tip 提问指南

+ 问题描述

写下详细的问题描述，包括所有有助于他人回答您问题的信息（例如实验过程、条件和相关图像等）。

post 发布问题
0 Q&A
pen 提交稿件