Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis seedling
拟南芥中丙二醛、叶绿素脯氨酸、可溶性糖和谷胱甘肽含量的分析
发布: 2013年07月20日第3卷第14期 DOI: 10.21769/BioProtoc.817 浏览次数: 35496
评审: Tie Liu
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参见作者原研究论文
The authors used this protocol in:
Jul 2012
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Abstract
The protocol has four sub-protocols, which are about the measurement of malondialdehyde, chlorophyll proline, soluble sugar, and glutathione content, respectively, in Arabidopsis seedling by using spectrophotometer. These methods are simple, effective and reproducible, which will help the researchers who are not familiar with these approaches, quickly get reliable results.
Keywords: Malondialdehyde (丙二醛)
Proline (脯氨酸)
Sugar (糖)
Glutathione (谷胱甘肽)
Arabidopsis (拟南芥)
I. Measurement of Malondialdehyde
Materials and Reagents
- Thiobarbituric acid (TBA) (Sigma-Aldrich, catalog number: T5500 )
- Trichloroacetic acid (TCA) (Sigma-Aldrich, catalog number: T9159 )
- Malondialdehyde (MDA) (BOC Sciences, catalog number: 542-78-9 )
Equipment
- Centrifuge
- Spectrophotometer
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Zhang, Z. and Huang, R. (2013). Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis seedling. Bio-protocol 3(14): e817. DOI: 10.21769/BioProtoc.817.
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分类
植物科学 > 植物生物化学 > 其它化合物
生物化学 > 糖类 > 葡萄糖
生物化学 > 其它化合物 > 叶绿素
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