拟南芥中丙二醛、叶绿素脯氨酸、可溶性糖和谷胱甘肽含量的分析

Zhijin  Zhang; Rongfeng Huang

doi:10.21769/BioProtoc.817

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Peer-reviewed

Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis seedling

拟南芥中丙二醛、叶绿素脯氨酸、可溶性糖和谷胱甘肽含量的分析

Zhijin Zhang

Rongfeng Huang email

发布: 2013年07月20日第3卷第14期 DOI: 10.21769/BioProtoc.817 浏览次数: 33668

评审: Tie Liu

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The authors used this protocol in:

Cover of The Plant Journal, featuring study using the protocol.

Jul 2012

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Abstract

The protocol has four sub-protocols, which are about the measurement of malondialdehyde, chlorophyll proline, soluble sugar, and glutathione content, respectively, in Arabidopsis seedling by using spectrophotometer. These methods are simple, effective and reproducible, which will help the researchers who are not familiar with these approaches, quickly get reliable results.

Keywords: Malondialdehyde (丙二醛)

I. Measurement of Malondialdehyde

Materials and Reagents

Thiobarbituric acid (TBA) (Sigma-Aldrich, catalog number: T5500 )
Trichloroacetic acid (TCA) (Sigma-Aldrich, catalog number: T9159 )
Malondialdehyde (MDA) (BOC Sciences, catalog number: 542-78-9 )

Equipment

Centrifuge
Spectrophotometer

Procedure

English

中文翻译

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如何引用

Zhang, Z. and Huang, R. (2013). Analysis of Malondialdehyde, Chlorophyll Proline, Soluble Sugar, and Glutathione Content in Arabidopsis seedling. Bio-protocol 3(14): e817. DOI: 10.21769/BioProtoc.817.