After official publication in Bio-protocol (https://bio-protocol.org/e5018), we received a question regarding our publication titled "Flow Cytometry Analysis of Microglial Phenotypes in the Murine Brain During Aging and Disease" (DOI: 10.21769/BioProtoc.5018) that highlighted a significant typo in our writing. The published paper states, "Prepare Enzyme Mix 2: Buffer Y (20 μL/sample) + Enzyme A (100 μL/sample)." The volume of Enzyme A should read 10 μL/sample and not 100 μL/sample. This volume has been corrected in the updated version along with other minor edits that were made while reviewing to improve clarity of the protocol. Changes are tracked in the attached version linked here.

Supplementary information

The following supporting information can be downloaded here.