这是一则更正通知, 查看更正后的实验方案。

Correction Notice: Isolation of Epithelial and Stromal Cells from Colon Tissues in Homeostasis and Under Inflammatory Conditions

更正:在稳态和炎症条件下从结肠组织中分离上皮细胞和基质细胞

 (*contributed equally to this work) 发布: 2024年05月05日 DOI: 10.21769/BioProtoc.4990 浏览次数: 710

评审: Anonymous reviewer(s)

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After official publication in Bio-protocol (https://bio-protocol.org/e4825), we realized there are a few of mistakes in the volumes provided in the Recipe section.

The following corrections have been made to the recipes tables:

Recipes

  1. Epithelial cell solution
    HBSS (Quantity): instead of 11,250 μL --> 13,950 μL

  2. Epithelial wash buffer
    Glutamax (Final concentration): instead of 10 mM --> 2 mM

  3. Epithelial Wash Buffer
    Adv-DMEM F12: instead of 4,900 uL--> 49,000 μL

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