Background

Wounding of C. elegans is being done by fungal infection of Drechmeria coniospora (Pujol et al., 2008), micro-injection needle (Xu and Chisholm, 2014), femtosecond laser treatment (Chung et al., 2006) and micrometer-scale fine glass shards (Zhang et al., 2015). Most of these existing protocols involves individual wounding of worms in order to observe the roles of interested molecular players upon injury and during the process of healing. Existing methods have provided loads of new and useful insights from the mechanism of healing in C. elegans which was also proven to be extrapolatable to higher mammalian models. Nevertheless, the methods were inadequate to obtain large wound population in a shortframe of time and hence the further utility of high throughput alaytical techniques in G, P and M studies becomes tedious. In the era where high throughput analyses become the future of research, a new alternate protocol is required to obtain large wound population in single stretch at quick frame of time. Incidentally, glass wool wounding protocol described over here was established (Pooranachithra et al., 2019) inspired by the protocol described by Zhang et al., (2015). In addition to high throughput analytical techniques, the method was also found to be reliable for high throughput screening of active therapeutics for improved wound healing.