Isolation of Cancer Epithelial Cells from Mouse Mammary Tumors
小鼠乳腺肿瘤上皮细胞的分离
发布: 2013年02月05日第3卷第3期 DOI: 10.21769/BioProtoc.326 浏览次数: 15691
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参见作者原研究论文
The authors used this protocol in:
May 2012
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Abstract
The isolation of cancer epithelial cells from mouse mammary tumor is accomplished by digestion of the solid tumor. Red blood cells and other contaminates are removed using several washing techniques such that primary epithelial cells can further enriched. This procedure yields primary tumor cells that can be used for in vitro tissue culture, fluorescence-activated cell sorting (FACS) and a wide variety of other experiments (Lo et al., 2012).
Materials and Reagents
- Bovine serum albumin (Sigma-Aldrich, catalog number: A9576 )
- Sterile Dulbecco’s phosphate buffered saline (PBS) (Sigma-Aldrich, catalog number: D8537 )
- NH4Cl solution (StemCell Technologies, catalog number: 07850 )
- Scalpel or Razor Blade
- EasySep Negative Selection Kit (StemCell Technologies, catalog number: 19757 )
- Accutase (ITC, catalog number: AT104 )
- Collagenase (StemCell Technologies, catalog number: 07912 )
- Penicillin/Streptomycin (Life Technologies, InvitrogenTM, catalog number: 10378-016 )
- Dulbecco’s Modified Eagle Medium/F12 (Sigma-Aldrich, catalog number: D8437 )
Equipment
- Swing bucket centrifuge
- Incubated shaker
- EasySep Magnet (StemCell Technologies, catalog number: 18000 )
- Sterile Nylon Mesh 40 micrometer cell strainer (BD Biosciences, Falcon®, catalog number: 352340 )
- 50 ml conical tube
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Johnson, S., Chen, H. and Lo, P. (2013). Isolation of Cancer Epithelial Cells from Mouse Mammary Tumors. Bio-protocol 3(3): e326. DOI: 10.21769/BioProtoc.326.
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分类
癌症生物学 > 通用技术 > 细胞生物学试验 > 细胞分离和培养
细胞生物学 > 细胞分离和培养 > 细胞分离
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