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Testing for Assortative Mating by Diet in Drosophila melanogaster

通过饮食控制测定黑腹果蝇的选型交配

PL Philip T Leftwich
TC Tracey Chapman

发布: 2018年10月20日第8卷第20期 DOI: 10.21769/BioProtoc.3057 浏览次数: 5692

评审: Qin TangAnonymous reviewer(s)

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The authors used this protocol in:

Cover of Proceedings of the National Academy of Sciences of the United States of America, featuring study using the protocol.

Nov 2017

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Abstract

Experimental studies of the evolution of reproductive isolation in real time are a powerful way to reveal the way that fundamental processes, such as mate choice, initiate divergence. Mate choice, while frequently described in females, can occur in either sex, and can be affected by the genetics or environment of an individual. Here we describe simple protocols for assessing mating outcomes in fruit flies, which in this context can be used to assess reproductive isolation derived from rearing on different diets over multiple generations.

Keywords: Ecological adaptation (生态适应) search Diet (饮食) search Assortative mating (选型交配) search Reproductive isolation (生殖隔离) search Drosophila (果蝇) search Mate choice (配偶选择) search Behavioural ecology (行为生态学) search

Background

Drosophila melanogaster is an important model for studying sexual selection, of which mate choice is a central component. Mate choice can be effected by many different variables such as direct and indirect genetic effects, environment and nutrition. This protocol was implemented in the previously published study (Leftwich et al., 2017). In that study, this assay was combined with microbiome characterization and manipulation to assess the impact of splitting a single population into two isolated groups reared on different diets over multiple generations.

Materials and Reagents

  1. Flystuff Drosophila tube polypropylene (Scientific Laboratory Supplies, Flystuff, catalog number: FLY1318 )
  2. Flystuff Cotton balls (Scientific Laboratory Supplies, Flystuff, catalog number: large for bottles FLY1200 and small for vials FLY1028 )
  3. Fly morgue (Plastic Funnel and Plastic Beaker)
  4. Thin paintbrush
  5. Dissecting needle (Watkins & Doncaster, catalog number: D416 )
  6. Pooter (homemade) (3 ml graduated Pasteur pipette, 6 mm rubber tubing, muslin)
    The plastic pipette is connected to the rubber tubing with muslin acting as a physical barrier between pipette and tube. Individual flies may be collected into the pipette by inhaling gently through the rubber tubing. 
  7. Polystyrene Petri dishes, 90 mm (Fisher Scientific, FisherbrandTM, catalog number: 12654785 )
  8. Filter paper, 90 mm, QL 100 (Fisher Scientific, FisherbrandTM, catalog number: 11566873 )
  9. Parafilm
  10. Drosophila melanogaster – stock lines Oregon-R and Dahomey (Bloomington Stock Center)
  11. Carbon Dioxide, Industrial Grade, BOC, 40-VK
  12. Fly food ingredients (store in a cool, dry place to maximize shelf life, do not keep for more than 12 months)
  13. Active Dried Yeast, 500 g (BakeryBits, catalog number: BB-1606 )
  14. Molasses 
  15. Agar (ForMedium, catalog number: AGA01 )
  16. Cornmeal/Maize Flour
  17. Sugar
  18. Starch (Fisher Scientific, FisherbrandTM, catalog number: S/7960/60 )
  19. Inactivated Brewer's Yeast
  20. Propionic Acid (Sigma-Aldrich, catalog number: 402907 )
  21. Nipagin M (Sigma-Aldrich, catalog number: H3547 )
  22. Ethanol absolute (Thermo Fisher Scientific, catalog number: E/0600DF/C17 )
  23. Red Grape Juice (BTP Drewitt Ltd.)
  24. Sugar yeast agar (SYA) medium (see Recipes)
  25. Cornmeal molasses yeast (CMY) medium (see Recipes)
  26. Starch medium (see Recipes)
  27. Grape juice plates (Purps) (see Recipes)

Equipment

  1. Flystuff Drosophila Glass Stock Bottle (Scientific Laboratory Supplies, Flystuff, catalog number: FLY1086 )
  2. Sharp scalpel
  3. Flystuff Benchtop Flow Buddy System with Fly Pad and Gun (Scientific Laboratory Supplies, Flystuff, catalog number: FLY1010 )
  4. Stereomicroscope (Leica Microsystems, model: Leica MZ75 )
  5. Watson Marlow Food Dispenser (Watson-Marlow Fluid Technology Group, catalog number: 520Di )
  6. Embryo collection cage Large (Scientific Laboratory Supplies, Flystuff, catalog number: FLY1214 )

Software

  1. R v3.3.2 (R Core Team, 2017)
  2. JMating v1.0 (Carvajal-Rodriguez and Rolan-Alvarez, 2006)

Procedure

English
中文翻译

文章信息

版权信息

© 2018 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Leftwich, P. T. and Chapman, T. (2018). Testing for Assortative Mating by Diet in Drosophila melanogaster. Bio-protocol 8(20): e3057. DOI: 10.21769/BioProtoc.3057.

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分类

神经科学 > 行为神经科学 > 实验动物模型 > 其它

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