Materials and Reagents

1. Murashige & Skoog medium including vitamins (Duchefa Biochemie, catalog number: M0222 )
   
2. Sucrose
   
3. MES (Sigma-Aldrich, catalog number: M8250 )
   
4. ddH₂O
   
5. KOH
   
6. Gelrite^TM (Duchefa Biochemie, catalog number: G1101.5000 )
   
7. Soil composed of blocking compost (Plantaflor^® Presstopfsubstrat) and sand in a 3:1 proportion
   
8. KNO₃
   
9. NH₄NO₃
   
10. K₂HPO₄
    
11. KH₂PO₄
    
12. Ca(NO₃)₂^.4H₂O
    
13. MgSO₄₂^.7H₂O
    
14. FeSO₄₂^.7H₂O
    
15. Kelamix
    
16. Fertilization solution: modified Hoagland’s solution (Johnson et al., 1957) diluted 1/60 (see Recipes)
    
17. 2MS medium (see Recipes)
    

Equipment

1. Plant growth chamber
   
2. Laminar flow hood
   
3. Sterile forceps
   
4. Glass jars, glass tubes or plastic containers suitable for in vitro growth
   Note: Equipments 1-4 is not required if plants are grown from tubers in soil.
   
5. Sterile scalpels
   
6. Pots
   
7. Stakes
   
8. Plant twist ties (e.g. Garden Odyssey, catalog number: T001 )
   
9. Beaker
   
10. Paper surgical tape (e.g. 3 M Micropore medical tape, catalog number: 1530-0 )
    
11. Transparent plastic bags
    
12. Adhesive tape
    

Procedure