Materials and Reagents

1. 1.5 ml Eppendorf tubes
   
2. Double-sided tape (NICHIBAN, catalog number: NW-5 )
   
3. Glass slide (Matsunami Glass, catalog number: S1126 )
   
4. 18 x 18 mm coverslip (thickness: 0.12-0.17 mm) (Matsunami Glass, catalog number: C018181 )
   
5. Pipette tips 
   
6. Filter paper
   
7. Salmonella SJW1103 strain (wild type for motility and chemotaxis) (Yamaguchi et al., 1984)
   
8. Salmonella MMHI0117 strain [∆fliH-fliI flhB(P28T)] (Minamino and Namba, 2008)
   
9. Bacto tryptone (BD, catalog number: 211705 )
   
10. Potassium dihydrogenphosphate (Wako Pure Chemical Industries, catalog number: 164-22635 )
    
11. Dipotassium hydrogenphosphate (Wako Pure Chemical Industries, catalog number: 164-04295 ) 
12. Bacto yeast extract (BD, catalog number: 212750 )
    
13. Bacto agar (BD, catalog number: 214010 )
    
14. Sodium chloride (Wako Pure Chemical Industries, catalog number: 192-13925 )
    
15. T-broth (TB) (see Recipes)
    
16. L-broth agar plate (see Recipes)

Equipment

1. Selection of single channel pipettes (1,000 µl, 100 µl) (Gilson, model: P-1000 , P-100 )
2. Shaking incubator (30 °C, at 200 rpm) (TAITEC, model: BR-40LF )
3. Centrifuge (able to hold 1.5 ml tube, spin at 6,000 x g) (TOMY SEIKO, model: MX-305 )
4. Spectrophotometer (able to measure OD₆₀₀) (GE Healthcare, model: GeneQuant 1300 )
   Note: This product has been discontinued by the manufacturer. 
5. Phase contrast microscope (Olympus, model: CH40 )
   
   1. 40x objective lens
   2. CCD camera (Hamamatsu Photonics, model: C5405 )
6. Objective micrometer (10 µm/pitch)
7. Hard-disk video recorder (Panasonic, model: DMR-XP25V )
   

Software

1. Move-tr/2D (Library Co., Tokyo)
   
2. Microsoft Excel (Microsoft)

Procedure