Establishment of Patient-Derived Xenografts in Mice
在小鼠中建立患者源性异种移植物
发布: 2016年11月20日第6卷第22期 DOI: 10.21769/BioProtoc.2008 浏览次数: 15450
评审: Clara Lubeseder-MartellatoAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Jun 2015
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Abstract
Patient-derived xenograft (PDX) models for cancer research have recently attracted considerable attention in both the academy and industry (Hidalgo et al., 2014; Wilding and Bodmer, 2014). PDX models have been developed from different tumor types including lung cancer to improve the drug development process. These models are used for pre-clinical drug evaluation and can be used for the predictive results of clinical outcomes because they conserve original tumor characteristics such as heterogeneity, complexity and molecular diversity (Kopetz et al., 2012). Additionally, PDX model provides the potential tool for the personalized drug therapy. In this protocol, we present methods for the establishment of PDX in mice using primary tumor tissues from patients with small cell lung cancer (SCLC).
Materials and Reagents
- Sterile alcohol prep pads (Covidien, catalog number: 6818 )
- Petri dishes (Corning, Falcon®, catalog number: 353003 )
- Tissue adhesive (3M, catalog number: 1469SB )
- 6 weeks old SCID mice (Charles River Laboratories International, catalog number: 236 ) or athymic nude mice (Envigo, catalog number: 069 )
- Ketamine hydrochloride/xylazine hydrochloride solution (Sigma-Aldrich, catalog number: K113 )
- Phosphate-buffered saline (PBS) (Mediatech, catalog number: 21-040-CV )
- 70% ethanol (Decon Labs, catalog number: 2401 )
Equipment
- Stainless steel sterile scalpels (Integra LifeSciences, Miltex®, catalog number: 4-423 )
- Operating scissors (Sklar Surgical Instruments, catalog number: 13-1045 )
- Micro dissecting forceps (Roboz Surgical Instrument, catalog number: RS-5135 )
- Biological safety cabinet (Labconco, catalog number: 3460001 )
- CO2 chamber in animal facility
- Heating pad (Sunbeam Products, catalog number: 000765-500-000 )
Procedure
文章信息
版权信息
© 2016 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Park, D., Wang, D., Chen, G. and Deng, X. (2016). Establishment of Patient-Derived Xenografts in Mice. Bio-protocol 6(22): e2008. DOI: 10.21769/BioProtoc.2008.
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分类
癌症生物学 > 侵袭和转移 > 动物模型 > 细胞侵袭
癌症生物学 > 通用技术 > 肿瘤形成
免疫学 > 动物模型 > 小鼠
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