Aorta Ring Assay
主动脉环试验
发布: 2016年07月05日第6卷第13期 DOI: 10.21769/BioProtoc.1856 浏览次数: 15622
评审: Letizia De ChiaraAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Jun 2015
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Abstract
Angiogenesis is the nature and pathological process of blood vessel growth from pre-existing vascular buds. It plays an important role in cancer and cardiovascular disease. The aorta ring assay is an approach to study angiogenesis. In this experiment, we used the aorta of rat as the study material, cleaned the surrounding tissue of aorta and cut it into 1 mm long rings. Next, the rings were cultured in growth factor-reduced matrigel polymerized at 37 °C. Angiogenesis was assessed at 7 days by using an inverted microscope platform.
Materials and Reagents
- 10 cm dish (Thermo Scientific, catalog number: 172931 )
- 24 well plates (Thermo Scientific, catalog number: 142475 )
- 2 ml and 5 ml disposable sterilized syringe
- Sprague-Dawley 4-week old rat (Zhejiang Chinese Medical University Animal Center)
- Growth factor-reduced matrigel (Corning, catalog number: 354230 )
- Dulbecco’s Modification of Eagle’s Medium with 1 g/L glucose glutamine & sodium pyruvate (Mediatech, catalog number: 10-014-CV )
- Fetal Bovine Serum (Biological Industries, catalog number: 04-001-1A )
- Phosphate buffer saline (PBS) (Shanghai ji’nuo, catalog number: GNM 20012 )
- Chloral hydrate (Guoyao chemical reagent co. LTD, catalog number: 30037517 )
- 70% ethanol
- Medium (see Recipes)
- 4% chloral hydrate (see Recipes)
Equipment
- Surgical scissors, scalpel and tweezers
- Ruler
- 37 °C, 5% CO2 cell culture incubator (Thermo Fisher Scientific, catalog number: 51026334 )
- Inverted microscope (Leica Microsystems, model: DMi1 )
- Electronic scale
Software
- Image-Pro Plus 6.0
Procedure
文章信息
版权信息
© 2016 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Jin, J., Hu, X., Zhang, L. and Wang, J. (2016). Aorta Ring Assay. Bio-protocol 6(13): e1856. DOI: 10.21769/BioProtoc.1856.
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分类
发育生物学 > 细胞生长和命运决定 > 血管生成
细胞生物学 > 组织分析 > 组织分离
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