小鼠脑干听觉区反应测试

Omar Akil; A. E. Oursler; Kevin Fan; Lawrence R. Lustig

doi:10.21769/BioProtoc.1768

Improve Research Reproducibility A Bio-protocol resource

提交稿件
订阅
CN
- EN - English
- CN - 中文

Peer-reviewed

Mouse Auditory Brainstem Response Testing

小鼠脑干听觉区反应测试

发布: 2016年03月20日第6卷第6期 DOI: 10.21769/BioProtoc.1768 浏览次数: 17130

评审: Oneil G. BhalalaPascal Fossat Anonymous reviewer(s)

下载 PDF

Q&A

引用

Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of The Journal of Neuroscience, featuring study using the protocol.

Feb 2015

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

相关实验方案

成年小鼠人工吸气方法

Thomas P. Eiting and Matt Wachowiak

2018年09月20日 5473 阅读

用于成年小鼠脑组织的细胞特异性CRISPR基因编辑的病毒载体的构建

Hiroshi Yamaguchi and Luis de Lecea

2019年08月20日 5859 阅读

可卡因调节小鼠位置偏爱研究

MaryElizabeth J. Simkevich [...] André O. White

2020年04月20日 4352 阅读

Abstract

The auditory brainstem response (ABR) test provides information about the inner ear (cochlea) and the central pathways for hearing. The ABR reflects the electrical responses of both the cochlear ganglion neurons and the nuclei of the central auditory pathway to sound stimulation (Zhou et al., 2006; Burkard et al., 2007). The ABR contains 5 identifiable wave forms, labeled as I-V. Wave I represents the summated response from the spiral ganglion and auditory nerve while waves II-V represent responses from the ascending auditory pathway. The ABR is recorded via electrodes placed on the scalp of an anesthetized animal. ABR thresholds refer to the lowest sound pressure level (SPL) that can generate identifiable electrical response waves.

This protocol describes the process of measuring the ABR of small rodents (mouse, rat, guinea pig, etc.), including anesthetizing the mouse, placing the electrodes on the scalp, recording click and tone burst stimuli and reading the obtained waveforms for ABR threshold values. As technology continues to evolve, ABR will likely provide more qualitative and quantitative information regarding the function of the auditory nerve and brainstem pathways involved in hearing.

Keywords: Mouse (小鼠)

Materials and Reagents

1 ml insulin syringe (Thermo Fisher Scientific, BD, catalog number: 22-253-260 )
Precision Glide Needles (30 G x 1/2) (BD, catalog number: 305106 )
Disposable monopolar needle electrodes for electromyography (Rochester Electro-Medical, catalog number: 016386-0 )
Ketamine hydrochloride
1. Ketaset (Ketamine HCl Injection) (Drugs, catalog number: NDC-0856-2013-01 )
2. Henry Schein (100 mg/ml solution) (NSN, catalog number: 304401 )
Xylazine hydrochloride (Sedative and Analgesic solution) (20 mg/ml solution) (LLOYD, AnaSed INJECTION, catalog number: 139-236 )
Sterile bi-distilled water
Povidone-iodine 5% (Betadine) antiseptic-Microbicide solution (purchased from any pharmacy)
Dexamethasone ophthalmic ointment (TobraDex) (Alcon)
70% Ethanol (Surface disinfectant)
Mixture of ketamine hydrochloride and xylazine hydrochloride (see Recipes)

Equipment

Disposable monopolar needle electrodes for electromyography (Rochester Electro Medical, catalog number: 016386-0)
Computer with BioSig32 Windows Application Installed
TDT BioSigIII system (TDT)
TD speakers (TDT)
Sound proof chamber (Industrial Acoustics Company, Controlled Acoustical Environment)
Non-Electric Heating Pad (to maintain animal body temperature during the test) (Braintree Scientific, Deltaphase Isothermal Pad, model: 39DP )
CONAIR Moist Electric Heating Pad (to maintain animal body temperature during the recovery) (Amazon)
Rectal Probe for body temperature monitoring (Physitemp Instruments, model: Thermalert TH-5 physitemp )
Clean empty cages
Tweezers style 5 (Ted Pella, catalog number: 5665 )

Software

BioSig32 software (coming with the TDT BioSig III system)

Procedure

English

中文翻译

文章信息

版权信息

如何引用

Readers should cite both the Bio-protocol article and the original research article where this protocol was used:

Akil, O., Oursler, A. E., Fan, K. and Lustig, L. R. (2016). Mouse Auditory Brainstem Response Testing. Bio-protocol 6(6): e1768. DOI: 10.21769/BioProtoc.1768.
Akil, O., Sun, Y., Vijayakumar, S., Zhang, W., Ku, T., Lee, C. K., Jones, S., Grabowski, G. A. and Lustig, L. R. (2015). Spiral ganglion degeneration and hearing loss as a consequence of satellite cell death in saposin B-deficient mice. J Neurosci 35(7): 3263-3275.