测试紫外线辐照对水稻细菌性谷枯病菌存活的影响

Hari Sharan Karki; Jong Hyun Ham

doi:10.21769/BioProtoc.1755

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Peer-reviewed

Testing the Effect of UV Radiation on the Survival of Burkholderia glumae

测试紫外线辐照对水稻细菌性谷枯病菌存活的影响

HK Hari Sharan Karki

Jong Hyun Ham email

发布: 2016年03月05日第6卷第5期 DOI: 10.21769/BioProtoc.1755 浏览次数: 9963

评审: Zhaohui LiuAnonymous reviewer(s)

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Cover of Molecular Plant Pathology, featuring study using the protocol.

Dec 2014

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Abstract

Burkholderia glumae (B. glumae) is becoming a serious threat in the major rice producing areas of the world. It was reported that Burkholderia spp., including B. glumae, are adapted to a wide range of ecological niches. Different bacterial strains show different levels of UV tolerance which may be due to the presence of different protection mechanisms. Previously we reported that pigment producing strains of B. glumae are more tolerant to UV radiation than non-pigmented strains. Here, we describe the protocol of UV tolerance assay for B. glumae in different exposure times. Using this protocol, we can calculate the survival rate of B. glumae strains, as well as other bacterial species, in exposure to UV radiation.

Materials and Reagents

Microcentrifuge tubes
Cuvettes (BrandTech Macro Methacrylate Cuvette) (Cole-Parmer, catalog number: 759081D )
Spreaders
Bacterial inoculation loops
A metal inoculation needle or sterile tooth picks
Strains of Burkholderia glumae (or other bacterial species)
Luria-Bertani broth (Sigma-Aldrich, catalog number: L3522 )
Bacteriological agar (Sigma-Aldrich, catalog number: A5306 )
Bacto Peptone (BD, Difco^TM, catalog number: 211677 )
Glucose (Sigma-Aldrich, catalog number: G5400 )
Casamino acid (Thermo Fisher Scientific, catalog number: BP1424 )
Nitrofurantoin (MP Biomedicals, catalog number: 155881 )
Luria-Bertani broth (LB) agar plates (see Recipes)
Casamino acid-peptone-glucose (CPG) agar plates (see Recipes)

Equipment

Laminar hood installed with a UV light bulb (The Baker Company, EdgeGard^®)
Spectrophotometer (Thermo Fisher Scientific, model: BioMate3 )
Incubator (GMI, New Brunswick Scientific, model: Innova 4230 )
Germicidal fluorescent bulb, 15 W (General Electric Company, model: G15T8 )

Procedure

English

中文翻译

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Karki, H. S. and Ham, J. H. (2016). Testing the Effect of UV Radiation on the Survival of Burkholderia glumae. Bio-protocol 6(5): e1755. DOI: 10.21769/BioProtoc.1755.