Calculation of Microorganism Lag Times as a Measure of Adaptative Capability between Different Growth Conditions

This protocol has been designed as a simple and efficient way to investigate microorganism adaptive capabilities (Enjalbert et al., 2015). It is performed using switch experiments in which cells are initially grown in the first condition (primary cultures), then rapidly switched to the second condition (secondary culture) without centrifugation or quenching. The measurement is based on the capacity of the secondary culture cells to resume growth. This protocol can be utilized for assessing metabolic or stress adaptation of microorganisms.

Lag (滞后)

Switch (开关)

Growth (生长)

Adaptation (适应)