Chlorophyll Content Assay to Quantify the Level of Necrosis Induced by Different R Gene/Elicitor Combinations after Transient Expression
通过测定叶绿素含量对瞬时表达后不同R基因/诱导子组合诱导产生的细胞坏死量进行定量测定
发布: 2015年12月05日第5卷第23期 DOI: 10.21769/BioProtoc.1670 浏览次数: 10099
评审: Feng LiAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Dec 2013
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Abstract
This assay can be used to rapidly and accurately quantify levels of leaf necrosis induced after transient expression of R genes and elicitor combinations (Harris et al., 2013). It is based on the inverse correlation between level of necrosis and chlorophyll content in leaf tissue. It is adapted from the calculations described by (Strain et al., 1971).
Materials and Reagents
- 1.5 ml tube
- Leaf discs
Note: Ensure that the leaf discs are fully submerged in the DMF solution. - N, N-Dimethylformamide (DMF) (Sigma-Aldrich, catalog number: D4551-250 ml )
Equipment
- Spectrophotometer (Spectronic Biomate3) (Thermo Fisher Scientific, catalog number: 335904P )
- Glass spectrophotometer cuvettes (Sigma-Aldrich, catalog number: Z276898 )
Note: Product Z276898 has been discontinued.
Procedure
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文章信息
版权信息
© 2015 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Harris, C. J. and Baulcombe, D. C. (2015). Chlorophyll Content Assay to Quantify the Level of Necrosis Induced by Different R Gene/Elicitor Combinations after Transient Expression. Bio-protocol 5(23): e1670. DOI: 10.21769/BioProtoc.1670.
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分类
植物科学 > 植物免疫 > 病害症状
植物科学 > 植物生理学 > 组织分析
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