乳腺癌细胞的悬滴聚集试验

Yong Teng

doi:10.21769/BioProtoc.1393

Improve Research Reproducibility A Bio-protocol resource

提交稿件
订阅
CN
- EN - English
- CN - 中文

Peer-reviewed

Hanging Drop Aggregation Assay of Breast Cancer Cells

乳腺癌细胞的悬滴聚集试验

Yong Teng

发布: 2015年02月05日第5卷第3期 DOI: 10.21769/BioProtoc.1393 浏览次数: 20397

评审: HongLok LungAnonymous reviewer(s)

下载 PDF

Q&A

引用

Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Oncogene, featuring study using the protocol.

Jan 2014

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

相关实验方案

体外流式粘附实验法分析转移癌细胞到内皮细胞的抗剪粘附性

Shin-Ae Kang [...] Takemi Tanaka

2016年02月20日 13432 阅读

利用成像和MATLAB分析研究细胞焦点粘附参数

Ling-Yea Yu [...] Feng-Chiao Tsai

2023年11月05日 935 阅读

小鼠原代周细胞的分离和培养

Tamara McErlain [...] Meera Murgai

2025年04月20日 1588 阅读

Abstract

Hanging drop assay can be used to investigate cell-cell cohesion and cell-substratum adhesion through generation of 3D spheroids under physiological conditions. It also can be used to elucidate the role of cell-cell or cell-ECM interactions in specifying spatial relationships between two (or more) different cell populations. This simple method requires no specialized equipment and provides a means of generating tissue-like cellular aggregates for measurement of biomechanical properties for molecular and biochemical analysis in a physiologically relevant model.

Keywords: Hanging drop (悬滴)

Cancer (癌症)

Aggregation (聚集)

Migration (迁移)

Materials and Reagents

Human breast cancer cell lines T-47D (ATCC, catalog number: ATCC^® HTB-133^TM ), MCF7 (ATCC, catalog number: ATCC^® HTB-22^TM )
Trypsin-EDTA (0.05%) (phenol red) (Life Technologies, catalog number: 25300062 )
Gibco^® RPMI media 1640 (Life Technologies, catalog number: 11875-085 )
Gibco^® PBS (Life Technologies, catalog number: 10010023 )
Fetal bovine serum (Life Technologies, catalog number: 16000-044 )
Gibco^® penicillin-streptomycin-glutamine (100x) (Life Technologies, catalog number: 10378-016 )
Complete medium (see Recipes)