Improve Research Reproducibility A Bio-protocol resource
Peer-reviewed
tooltip

Determination of Mitochondrial DNA Upon Drug Treatment

药物处理后的线粒体DNA测定

MP Michel Perron
Joy Y. Feng Joy Y. Feng

发布: 2015年01月20日第5卷第2期 DOI: 10.21769/BioProtoc.1377 浏览次数: 9292

评审: Vanesa Olivares-IllanaPia GiovannelliAnonymous reviewer(s)

download pdf PDF PDF
ask Q&A
引用
收藏
Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of Antimicrobial Agents and Chemotherapy, featuring study using the protocol.

Apr 2014

Advertisement

“科研可重复性与创新”系列网络研讨会
Special Issue on Protocols for Organoid Research
Technologies for RNA Detection
Presubmission Inquiry

实验方案合集

专注于特定技术或应用的、详细的、经过同行评审的实验方案合集

Cancer Research
Immunology
Developmental Biology
Microbiology
Neuroscience
Cell Imaging - A Special Collection for Cell Bio 2023
查看更多 more

相关实验方案

利用免疫荧光和Fiji软件定量分析辐照细胞中γH2AX焦点的实验方案

利用免疫荧光和Fiji软件定量分析辐照细胞中γH2AX焦点的实验方案

Lu Deng [...] Lingying Wu

2025年08月20日 3199 阅读

利用 IF-FISH 技术在小鼠脑神经元中检测端粒与 PML 或 γH2AX 焦点的共定位

利用 IF-FISH 技术在小鼠脑神经元中检测端粒与 PML 或 γH2AX 焦点的共定位

Anna Konopka

2025年11月05日 1757 阅读

用于监测复制叉推进、保护与重启的定量 DNA 纤维分析方法

用于监测复制叉推进、保护与重启的定量 DNA 纤维分析方法

Debanjali Bhattacharya and Ganesh Nagaraju

2026年02月05日 812 阅读

Abstract

Drug-induced mitochondrial injury can be caused by many different mechanisms including inhibition of mitochondrial DNA replication, transcription, translation, and altered protein function. Determination of the level of mitochondrial DNA relative to the nuclear DNA levels provides important information on potential mitochondrial toxicity.

Keywords: Mitochondrial toxicity (线粒体毒性) search Nucleoside analogs (核甘酸类似物) search Inhibitor of DNA synthesis (DNA合成抑制剂) search

Materials and Reagents

  1. HepG2 cells (ATCC, catalog number: HB 8065 )
  2. DMSO (cell culture grade) (Sigma-Aldrich, catalog number: D2650 )
  3. Phosphor-buffered saline (PBS) (Life Sciences, catalog number: 10010049 )
  4. QIAamp DNA mini kit (QIAGEN, catalog number: 51304 )
  5. TaqMan universal mastermix (Life Technologies, Applied Biosystems®, catalog number: 4352042 )
  6. β-actin Assay-on-Demand kit (Life Technologies, Applied Biosystems®, catalog number: 4331182 )
  7. Eagle’s minimum essential medium (Life Technologies, Gibco®, catalog number: 41090 )
  8. GlutaMAXTM
  9. Fetal bovine serum (FBS) (HyClone, catalog number: SH30071.03 )
  10. 100 units/ml penicillin, 100 units/ml streptomycin (Life Technologies, Gibco®, catalog number: 15140 )
  11. Sodium pyruvate (Life Technologies, Gibco®, catalog number: 11360 )
  12. Cells were cultured in Eagle’s minimum essential medium (see Recipes)
    Note: Cells were cultured in Eagle’s minimum essential medium.

Equipment

  1. 12-well plates (Corning, catalog number: 3513 )
  2. ABI Prism 7900HT Fast Real-Time PCR system (Life Technologies, Applied Biosystems®)

Procedure

English
中文翻译

文章信息

版权信息

© 2015 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Perron, M. and Feng, J. Y. (2015). Determination of Mitochondrial DNA Upon Drug Treatment. Bio-protocol 5(2): e1377. DOI: 10.21769/BioProtoc.1377.

ris ris Download Citation in RIS Format

分类

分子生物学 > DNA > DNA 损伤和修复

分子生物学 > DNA > DNA 定量

您对这篇实验方法有问题吗？

在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。

post 发布问题
0 Q&A
pen 提交稿件