血细胞凝集试验

Praveen  Papareddy; Martina  Kalle; Artur  Schmidtchen

doi:10.21769/BioProtoc.1247

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Peer-reviewed

Coagulation Assay

血细胞凝集试验

PP Praveen Papareddy email

MK Martina Kalle

AS Artur Schmidtchen

发布: 2014年10月05日第4卷第19期 DOI: 10.21769/BioProtoc.1247 浏览次数: 14949

评审: Fanglian HeAnonymous reviewer(s)

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参见作者原研究论文

The authors used this protocol in:

Cover of PLOS Pathogens, featuring study using the protocol.

Dec 2013

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Abstract

Clotting times can be measured by using citrate plasma. The intrinsic pathway of coagulation is measured by the activated partial thromboplastin time (aPTT), the extrinsic pathway of coagulation, monitored by measuring the prothrombin time (PT), and thrombin-induced fibrin-network formation (thrombin clotting time; TCT).

Materials and Reagents

Citrated plasma (fresh or frozen)
Eppendorf tubes
Coagulation reagents
1. Thrombin reagent (Technoclone, catalog number: 5100005 )
2. TriniCLOT PT Excel reagent (Trinity Biotech, catalog number: T1105 / T1106 )
3. DAPTTIN TC (Technoclone, catalog number: 5035060 )
30 mM CaCl₂ (freshly made)
Test agent (e.g. antimicrobial peptide LL-37)

Equipment

BD vacutainer^® plus blood collection tubes (BD, catalog number: 364305 )
Coagulometer (Mc10 Plus merlin medical)
Cuvettes and balls macro (merlin medical, catalog number: Z05100 )

Procedure

English

中文翻译

文章信息

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如何引用

Readers should cite both the Bio-protocol article and the original research article where this protocol was used:

Papareddy, P., Kalle, M. and Schmidtchen, A. (2014). Coagulation Assay. Bio-protocol 4(19): e1247. DOI: 10.21769/BioProtoc.1247.
Papareddy, P., Kalle, M., Sorensen, O. E., Malmsten, M., Morgelin, M. and Schmidtchen, A. (2013). The TFPI-2 derived peptide EDC34 improves outcome of gram-negative sepsis. PLoS Pathog 9(12): e1003803.