屏障功能试验

Tia  DiTommaso; Floris  Foijer

doi:10.21769/BioProtoc.1133

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Peer-reviewed

Barrier Function Assay

屏障功能试验

TD Tia DiTommaso

Floris Foijer email

发布: 2014年05月20日第4卷第10期 DOI: 10.21769/BioProtoc.1133 浏览次数: 10773

评审: Lin FangFanglian HeAnonymous reviewer(s)

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参见作者原研究论文

The authors used this protocol in:

Cover of Proceedings of the National Academy of Sciences of the United States of America, featuring study using the protocol.

Feb 2013

实验方案合集

Cell Imaging - A Special Collection for Cell Bio 2023

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Abstract

This protocol serves to determine the integrity of the barrier function of (murine) epidermis. Defects in the barrier function lead to dehydration and infection in neonatal mice/humans. One possible way to assess epidermal barrier integrity is by a dye penetration assay as described hereunder. This assay should be done on unfixed, untreated tissues (e.g. formaldehyde- or glutaraldehyde-fixed) or on whole mouse embryos (E18.5). This protocol was adapted from Hardman (1998).

Keywords: Skin integrity (皮肤的完整性)