Electrical Penetration Graph Recording (Whitefly)
电渗透图记录（粉虱）
发布: 2014年03月05日第4卷第5期 DOI: 10.21769/BioProtoc.1063 浏览次数: 12644
评审: Anonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
May 2013
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Abstract
Electrical penetration graph (EPG) was first developed by Mclean and Kinsey (1964) and later modified by Tjallingii (1978 and 1985). The EPG system turns a phloem-sucking insects and its host plant into part of an electrical circuit that is completed when the insect’s mouthparts penetrate the plant. The electrical signal is amplified by an amplifier and digitized by a converter. Fluctuations in voltage and electrical resistance are recorded and can be matched to specific feeding events. Current protocol is an efficient use of the EPG system to record whitefly feeding behavior on plants.
Materials and Reagents
- Plant (e.g., 2-3 true leaf stage)
- Newly-emerged (e.g., 2-5 d old) female whiteflies
Equipment
- Ice-bag or box
- Glass dish (Diameter = 6 cm)
- Gold wire (Diameter = 12.5 μm, http://www.epgsystems.eu/products.php)
- Silver glue (Vial of 2ml glue, http://www.epgsystems.eu/products.php)
- Faraday cage (self-made cage with10 mesh wire net, length = 60 cm, width = 60 cm, height = 70 cm)
- Insect-proof cage (self-made cage with 80 mesh nylon screening, length = 40 cm, width = 40 cm, height = 60 cm)
- Transparent tubes (self-made tube with bottle, 3 cm long, inner diameter = 5 mm)
- Small ice-chilled glass dish
- Giga-8 DC-EPG system with 109 Ohm input resistance (Wageningen University, http://www.epgsystems.eu/products.php)
- DI710-UL analogue-to-digital converter (DATAQ Instruments, http://www.epgsystems.eu/products.php)
- Computer
- Dissecting microscope
Software
- PROBE 3.4 software (Wageningen University, advanced version can be downloaded from http://www.epgsystems.eu/downloads.php)
Procedure
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文章信息
版权信息
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Liu, B., Zhou, X. and Zhang, Y. (2014). Electrical Penetration Graph Recording (Whitefly). Bio-protocol 4(5): e1063. DOI: 10.21769/BioProtoc.1063.
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分类
植物科学 > 植物免疫 > 病害生物测定
植物科学 > 植物生理学 > 组织分析
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