Improve Research Reproducibility A Bio-protocol resource
Peer-reviewed

Monocular Deprivation in Mice

小鼠单眼剥离试验

Ya-tang Li Ya-tang Li
XC Xiao-lin Chou
H Huizhong Whit Tao

发布: 2014年01月20日第4卷第2期 DOI: 10.21769/BioProtoc.1024 浏览次数: 9564

评审: Xuecai GeAnonymous reviewer(s)

download pdf PDF PDF
ask Q&A
引用
收藏
Cited by

参见作者原研究论文

The authors used this protocol in:

Cover of The Journal of Neuroscience, featuring study using the protocol.

Jul 2013

Presubmission Inquiry

实验方案合集

专注于特定技术或应用的、详细的、经过同行评审的实验方案合集

Cancer Research
Immunology
Developmental Biology
Microbiology
Neuroscience
Cell Imaging - A Special Collection for Cell Bio 2023
查看更多 more

Abstract

Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than three days (long term). Here we describe a detailed protocol for short-term and long-term monocular deprivation in mouse (Ma et al., 2013).

Materials and Reagents

  1. Cotton
  2. 75% Ethanol
  3. Ketamine hydrochloride (100 mg/ml) (Ketaject, Phoenix Pharmaceuticals, NDC number: 57319-542-02 )
  4. Xylazine hydrochloride (100 mg/ml) (AnaSed Injection, LLOYD, NADA number: 139-236 )
  5. Xylocaine (lidocaine hydrochloride) 2% Jelly (AstraZeneca, NDC number: 0186-0330-01 )
  6. Bacitracin zinc ointment (USP 500 U/g) (Fougera Pharmaceuticals, NDC number: 0168-0011-31 )
  7. Saline (0.9% sodium chloride injection USP)

Equipment

  1. Hood
  2. Autoclave
  3. Aluminum foil
  4. Dumont #5 forcep (Fine Science Tools, catalog number: 11254-20 )
  5. Dumont #3c forcep (Fine Science Tools, catalog number: 11231-20 )
  6. Cohan-Vannas spring scissor (Fine Science Tools, catalog number: 15000-02 )
  7. Fine scissor (Fine Science Tools, catalog number: 14060-09 )
  8. UNIFY Silk surgical suture [small (P-3) 13 mm, reverse cutting 3/8 circle needle. 4-0 18"/45 cm thread] (AD Surgical)
  9. Alcohol pad (PDI, catalog number: B603 )
  10. Gloves
  11. Microscope
  12. Illuminator (AmScope, catalog number: Model HL250-AY )
  13. Heating pad (Harvard Apparatus, catalog number: 507220F )

Procedure

English
中文翻译

文章信息

版权信息

© 2014 The Authors; exclusive licensee Bio-protocol LLC.

如何引用

Readers should cite both the Bio-protocol article and the original research article where this protocol was used:

  1. Li, Y., Chou, X. and Tao, H. W. (2014). Monocular Deprivation in Mice. Bio-protocol 4(2): e1024. DOI: 10.21769/BioProtoc.1024.
  2. Ma, W. P., Li, Y. T. and Tao, H. W. (2013). Downregulation of cortical inhibition mediates ocular dominance plasticity during the critical period. J Neurosci 33(27): 11276-11280.

    3. ris ris Download Citation in RIS Format

分类

神经科学 > 感觉和运动系统

您对这篇实验方法有问题吗？

在此处发布您的问题，我们将邀请本文作者来回答。同时，我们会将您的问题发布到Bio-protocol Exchange，以便寻求社区成员的帮助。

0/150

tip 提问指南

+ 问题描述

写下详细的问题描述，包括所有有助于他人回答您问题的信息（例如实验过程、条件和相关图像等）。

post 发布问题
0 Q&A
pen 提交稿件