Monocular Deprivation in Mice
小鼠单眼剥离试验
发布: 2014年01月20日第4卷第2期 DOI: 10.21769/BioProtoc.1024 浏览次数: 9855
评审: Xuecai GeAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
Jul 2013
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Abstract
Monocular deprivation is an experimental technique to study the ocular dominance plasticity during critical period (Hubel and Wiesel, 1963). Generally one eye of an animal is sutured during critical period, and the sutured eye is re-opened after either less than three days (short term) or more than three days (long term). Here we describe a detailed protocol for short-term and long-term monocular deprivation in mouse (Ma et al., 2013).
Materials and Reagents
- Cotton
- 75% Ethanol
- Ketamine hydrochloride (100 mg/ml) (Ketaject, Phoenix Pharmaceuticals, NDC number: 57319-542-02 )
- Xylazine hydrochloride (100 mg/ml) (AnaSed Injection, LLOYD, NADA number: 139-236 )
- Xylocaine (lidocaine hydrochloride) 2% Jelly (AstraZeneca, NDC number: 0186-0330-01 )
- Bacitracin zinc ointment (USP 500 U/g) (Fougera Pharmaceuticals, NDC number: 0168-0011-31 )
- Saline (0.9% sodium chloride injection USP)
Equipment
- Hood
- Autoclave
- Aluminum foil
- Dumont #5 forcep (Fine Science Tools, catalog number: 11254-20 )
- Dumont #3c forcep (Fine Science Tools, catalog number: 11231-20 )
- Cohan-Vannas spring scissor (Fine Science Tools, catalog number: 15000-02 )
- Fine scissor (Fine Science Tools, catalog number: 14060-09 )
- UNIFY Silk surgical suture [small (P-3) 13 mm, reverse cutting 3/8 circle needle. 4-0 18"/45 cm thread] (AD Surgical)
- Alcohol pad (PDI, catalog number: B603 )
- Gloves
- Microscope
- Illuminator (AmScope, catalog number: Model HL250-AY )
- Heating pad (Harvard Apparatus, catalog number: 507220F )
Procedure
文章信息
版权信息
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
- Li, Y., Chou, X. and Tao, H. W. (2014). Monocular Deprivation in Mice. Bio-protocol 4(2): e1024. DOI: 10.21769/BioProtoc.1024.
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Ma, W. P., Li, Y. T. and Tao, H. W. (2013). Downregulation of cortical inhibition mediates ocular dominance plasticity during the critical period. J Neurosci 33(27): 11276-11280.
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分类
神经科学 > 感觉和运动系统
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