Materials and Reagents

1. Tissue
   
2. OCT
   
3. 4% formaldehyde
   
4. PBS
   
5. Triton
   
6. Goat serum
   
7. BSA
   
8. Primary antibody: 53BP1 #NB 100-304 rabbit from Novus
   
9. Second antibody: goat anti-rabbit Alexa Fluor^® 488 Dye
   
10. Triton
    
11. Glycine
    
12. Mowiol 4-88 reagent (Calbiochem^®)
    
13. Formamide
    
14. Tris HCl, pH 7.4
    
15. Telomeric PNA probe (TelC-Cy3 from PANAGENE, catalog number: F1002-5 )
    
16. Tween-20
    
17. DAPI
    
18. Hybridization mixture (see Recipes)
    
19. Blocking reagent (Roche Diagnostics, catalog number: 11096176001 ) (see Recipes)
    
20. Wash solution I (see Recipes)
    
21. Wash solution II (see Recipes)
    

Equipment

1. Glass slide
   
2. Metal thermoblock
   
3. Humidified chamber
   

Procedure